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Effect of metal ion on the structure and function of LiPDF: The study of the fine structure around the metal site using XANES
Wang, Y; Chu WS(储旺盛); Yang FF(杨飞飞); Yu MJ(于梅娟); Zhao HF(赵海峰); Dong YH(董宇辉); Xie YN(谢亚宁); Wu ZY(吴自玉); Chu, WS; Yang, FF; Yu, MJ; Zhao, HF; Gong, WM; Dong, YH; Xie, YN; Wu, ZY
2010
发表期刊NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
卷号619期号:1-3页码:#REF!
通讯作者Chu, WS (reprint author), Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China.
摘要We used X-ray absorption near edge structure (XANES) spectroscopy to investigate the metal-dependent enzymatic activity of the peptide deformylase from Leptospira interrogans (LiPDF). Ab initio full multiple scattering calculations performed by MXAN are applied to obtain the local structure of the cobalt-containing LiPDF (Co-LiPDF) and zinc-containing LiPDF (Zn-LiPDF) around the metal sites in pH9.0 buffer solution. The result shows the cobalt-wat1 (the bond water molecule) distance of Co-LiPDF is 1.89 angstrom, much shorter than that of Zn-LiPDF, 2.50 angstrom. That is an essential factor for its low catalytic activity. (C) 2010 Elsevier B.V. All rights reserved.
文章类型Article; Proceedings Paper
关键词LiPDF BioXAS Metalloprotein
学科领域Instruments & Instrumentation; Nuclear Science & Technology; Physics; Spectroscopy
DOI10.1016/j.nima.2010.02.093
收录类别SCI
WOS类目Instruments & Instrumentation ; Nuclear Science & Technology ; Physics, Particles & Fields ; Spectroscopy
WOS记录号WOS:000280026300031
引用统计
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226258
专题多学科研究中心
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GB/T 7714
Wang, Y,Chu WS,Yang FF,et al. Effect of metal ion on the structure and function of LiPDF: The study of the fine structure around the metal site using XANES[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,2010,619(1-3):#REF!.
APA Wang, Y.,储旺盛.,杨飞飞.,于梅娟.,赵海峰.,...&Wu, ZY.(2010).Effect of metal ion on the structure and function of LiPDF: The study of the fine structure around the metal site using XANES.NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,619(1-3),#REF!.
MLA Wang, Y,et al."Effect of metal ion on the structure and function of LiPDF: The study of the fine structure around the metal site using XANES".NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT 619.1-3(2010):#REF!.
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