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Investigation of zinc-containing peptide deformylase from Leptospira interrogans by X-ray absorption near-edge spectroscopy
Li SJ(李树军); Chu WS(储旺盛); Hu TD(胡天斗); Xie YN(谢亚宁); Xian DC(冼鼎昌); Wu ZY(吴自玉); Li, SJ; Zhou, ZC; Chu, WS; Gong, WM; Benfatto, M; Hu, TD; Xie, YN; Xian, DC; Wu, ZY
2005
Source PublicationJOURNAL OF SYNCHROTRON RADIATION
Volume12Issue:1Pages:#REF!
Corresponding AuthorGong, WM (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China.
SubtypeArticle
AbstractPeptide deformylase (PDF, EC 3.5.1.27) is essential for the normal growth of eubacterium but not for mammalians. Recently, PDF has been studied as a target for new antibiotics. Its activity is strongly dependent on the bound metal ion. The crystallographic studies did not show any significant structural difference upon various bound metal ions. In this paper, X-ray absorption spectroscopy was employed to determine the local structure around the zinc ion of PDF from Leptospira interrogans in dry powder. XANES (X-ray absorption near-edge structure) calculations were performed and the local geometry of the active center was reconstructed successfully. By comparing with the crystal structure of an enzyme-product complex, the results from calculations show that a water molecule has moved towards the zinc ion and lies in the distance range to coordinate with the zinc ion weakly.
Keywordpeptide deformylase metalloproteinases catalytic mechanism BioXAS
Subject AreaInstruments & Instrumentation; Optics; Physics
DOI10.1107/S0909049504031413
Indexed BySCI
WOS SubjectInstruments & Instrumentation ; Optics ; Physics, Applied
WOS IDWOS:000225960900019
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Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/226230
Collection院士
多学科研究中心
Recommended Citation
GB/T 7714
Li SJ,Chu WS,Hu TD,et al. Investigation of zinc-containing peptide deformylase from Leptospira interrogans by X-ray absorption near-edge spectroscopy[J]. JOURNAL OF SYNCHROTRON RADIATION,2005,12(1):#REF!.
APA 李树军.,储旺盛.,胡天斗.,谢亚宁.,冼鼎昌.,...&Wu, ZY.(2005).Investigation of zinc-containing peptide deformylase from Leptospira interrogans by X-ray absorption near-edge spectroscopy.JOURNAL OF SYNCHROTRON RADIATION,12(1),#REF!.
MLA 李树军,et al."Investigation of zinc-containing peptide deformylase from Leptospira interrogans by X-ray absorption near-edge spectroscopy".JOURNAL OF SYNCHROTRON RADIATION 12.1(2005):#REF!.
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