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Distribution of selenium-containing proteins in human serum
Gao YX(高愈希); Gao, YX; Liu, YB; Deng, GL; Wang, ZJ
2004
发表期刊BIOLOGICAL TRACE ELEMENT RESEARCH
卷号100期号:2页码:#REF!
通讯作者Wang, ZJ (reprint author), Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China.
摘要Selenium-containing proteins in human serum of four volunteers in Beijing were separated and purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Selenium contents in the proteins were quantified by high-performance liquid chromatography (HPLC) coupled with a fluorescence detector (FLD) after pretreatment with a microwave digestion system and derivatization with 2,3-diaminonaphthalene (DAN). Five selenium-containing proteins with apparent molecular weights (MWs) of 68+/-3, 57.5+/-2.5, 47+/-2, 41+/-1, and 21+/-1 kDa, respectively, were detected. By comparison with known data on serum selenium-containing proteins, the 68+/-3 kDa protein should belong to albumin, which took 6.3-9.8% of the total serum Se. The 57.5+/-2.5 kDa protein should be selenoprotein P and the 47+/-2 kDa protein was believed to be an isoform of selenoprotein P. The sum of Se in selenoprotein P and its isomer took about 41.1-69.3% and was the major form of human serum selenium. The 21+/-1 kDa protein should be plasma glutathione peroxidase (p-GPx) and its Se content was about 21.1-24.3%. Also, protein of 41+/-1 kDa should be a selenium-containing protein that, to our best knowledge, was reported for the first time. The Se percentage in this protein corresponded to 12.6-20.4% of total human serum Se.
文章类型Article
关键词selenium selenoprotein human serum
学科领域Biochemistry & Molecular Biology; Endocrinology & Metabolism
DOI10.1385/BTER:100:2:105
收录类别SCI
WOS类目Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS记录号WOS:000223448900002
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226179
专题多学科研究中心
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GB/T 7714
Gao YX,Gao, YX,Liu, YB,et al. Distribution of selenium-containing proteins in human serum[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2004,100(2):#REF!.
APA 高愈希,Gao, YX,Liu, YB,Deng, GL,&Wang, ZJ.(2004).Distribution of selenium-containing proteins in human serum.BIOLOGICAL TRACE ELEMENT RESEARCH,100(2),#REF!.
MLA 高愈希,et al."Distribution of selenium-containing proteins in human serum".BIOLOGICAL TRACE ELEMENT RESEARCH 100.2(2004):#REF!.
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