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Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry
Shi JW(史俊稳); Feng WY(丰伟悦); Wang M(王萌); Zhang F(张芳); Li B(李柏); Wang B(汪冰); Zhu MT(朱墨桃); Chai ZF(柴之芳); Shi, JW; Feng, WY; Wang, M; Zhang, F; Li, B; Wang, B; Zhu, MT; Chai, ZF
刊名ANALYTICA CHIMICA ACTA
2007
卷号583期号:1页码:#REF!
关键词mercury-containing proteins stable isotopic tracer size-exclusion chromatography reverse isotope dilution ICP-MS
学科分类Chemistry
DOI10.1016/j.aca.2006.09.040
通讯作者Feng, WY (reprint author), Chinese Acad Sci, Inst High Energy Phys, Lab Bioenvironm Hlth Sci Nanoscale Mat, Beijing 100049, Peoples R China.
文章类型Article
英文摘要In order to investigate trace mercury-containing proteins in maternal rat and their offspring, a method of enriched stable isotopic tracer (Hg-196 and Hg-198) combined with size-exclusion chromatography (SEC) coupled to inductively coupled plasma-isotope dilution mass spectrometry (ICP-IDMS) was developed. Prior to the analysis, Hg-196- and Hg-198-enriched methylmercury was administrated to the pregnant rats. Then the mercury-containing proteins in serum and brain cytosol of the dam and pup rats were separated by size-exclusion columns and the mercury was detected by ICP-MS. The ICP-MS spectrogram of the tracing samples showed significantly elevated Hg-196 and Hg-198 isotopic signals compared with the natural ones, indicating that the detection sensitivity could be increased by the tracer method. The contents of mercury in chromatographic fractions of the dam and pup rat brain cytosol were quantitatively estimated by post-column reverse ID-ICP-MS. The quantitative speciation differences of mercury in brain cytosol between the dam and pup rats were observed, indicating that such studies could be useful for toxicological estimation. Additionally, the isotopic ratio measurement of Hg-198/Hg-202 in the tracing samples could be used to identify the artifact mercury species caused in the analytical procedure. The study demonstrates that the tracer method combined with high-performance liquid chromatography (HPLC)-ICP-IDMS could provide reliably qualitative and quantitative information on mercury-containing proteins in organisms. (c) 2006 Elsevier B.V. All rights reserved.
类目[WOS]Chemistry, Analytical
收录类别SCI
WOS记录号WOS:000243705500012
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226025
专题院士_期刊论文
中国科学院高能物理研究所_实验物理中心
中国科学院高能物理研究所_多学科研究中心
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Shi JW,Feng WY,Wang M,et al. Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2007,583(1):#REF!.
APA 史俊稳.,丰伟悦.,王萌.,张芳.,李柏.,...&Chai, ZF.(2007).Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry.ANALYTICA CHIMICA ACTA,583(1),#REF!.
MLA 史俊稳,et al."Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry".ANALYTICA CHIMICA ACTA 583.1(2007):#REF!.
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