Synchrotron X-ray diffraction and differential scanning calorimetry were used to study the effect of urea solutions on the phase behavior of Dioleoylglycero-phosphatidylethanolamine (DOPE). It was found that urea could stabilize lamellar liquid-crystalline phase at the expense of lamellar-gel phase and non-bilayer inverted hexagonal phase. The repeat distance of DOPE multilamellar structure increases with the increasing of urea concentration. The molecular mechanisms are discussed in terms of the possible hydrogen bonds between urea and the phospholipid head groups and the Hofmeister effect of urea, where urea is taken as a water-structure breaker. In addition, X-ray diffraction pattern of a wet rat tail collagen fiber was collected and was used as calibration standard at small-angle diffraction stations.
Yu, ZW,Feng, Y,Wang, J,et al. Study of the effect of urea on the phase transition of phospholipid by synchrotron X-ray diffraction[J]. HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,2001,25:#REF!.
Yu, ZW,Feng, Y,Wang, J,Dong, BZ,Quinn, PJ,&董宝中.(2001).Study of the effect of urea on the phase transition of phospholipid by synchrotron X-ray diffraction.HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,25,#REF!.
Yu, ZW,et al."Study of the effect of urea on the phase transition of phospholipid by synchrotron X-ray diffraction".HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION 25(2001):#REF!.