Wang, B (reprint author), CAS, Inst High Energy Phys, Lab Bioenvironm Hlth Sci Nanoscale Mat, Beijing 100049, Peoples R China.
The contents and micro distribution of iron and other trace elements in the olfactory bulb after intranasal instillation were measured by synchrotron radiation X-ray fluorescence analysis (SRXRF). The mice were intranasally instilled of fine iron oxide particles (60-200nm) at a dose of 40mg/kg b ody weight. Two weeks later, the content of iron in the olfactory bulb increased 20% compared with the control. The elevated iron was predominately concerned in the olfactory nerve (ON), granular layer (Gl) and anterior olfactory nucleus, external part (AOE), which indicated that fine iron oxide was transported to Cl via primary olfactory nerve and further translocated in AOE via secondary olfactory nerve. Moreover, in the experiment group, the levels of Ca increased 12% and Zn decreased 17%. Similarly to Fe, the higher Ca concentration was dominantly accumulated in the ON and Gl layers as well. Although the average Cu content in the control and experimental group was similar, the distribution of Cu was obviously changed due to the exposure of fine Fe2O3 particles. In the control, Cu was primarily concerned in anterior olfactory nucleus, lateral part (AOL), AOE, accessory olfactory bulb (AOB) and granule cell layer of the accessory olfactory bulb (GrA), while the Cu content in the experimental group was significantly higher in the ON, Gl and EPI layers. In conclusion, the changes of Fe, Ca, Zn, Cu levels must be, to some extent, correlate with the oxidative stress and neurodegenerative diseases.
Wan DY,He Q,Jia QJ,et al. Microtranslocation of trace element in the olfactory bulb of mice after intranasal instillation of fine oxide particle by synchrotron radiation x-ray fluorescence[J]. HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,2005,29:#REF!.
万冬云.,何庆.,贾全杰.,张仁刚.,张辉.,...&Xie, YN.(2005).Microtranslocation of trace element in the olfactory bulb of mice after intranasal instillation of fine oxide particle by synchrotron radiation x-ray fluorescence.HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,29,#REF!.
万冬云,et al."Microtranslocation of trace element in the olfactory bulb of mice after intranasal instillation of fine oxide particle by synchrotron radiation x-ray fluorescence".HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION 29(2005):#REF!.