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Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach
Hong, F; Liu, C; Zheng, L; Wang, XF; Wu, K; Song, WP; Lu, SP; Tao, Y; Zhao, GW; Tao Y(陶冶)
2005
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
Volume48Issue:1Pages:#REF!
Corresponding AuthorHong, F (reprint author), Suzhou Univ, Coll Life Sci, Suzhou 215006, Peoples R China.
SubtypeArticle
AbstractThe complex of Rubisco and Rubisco activase from LaCl3-, CeCl3- treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only large and small subunits (55 kD, 14.4 kD) of Rubisco, but also two large subunits of 45 kD and 41 kD near the large subunits of Rubisco. Native-PAGE shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only a band of Rubisco (560 0), but also a band of about 1100 0, about twice distant from Rubisco, which might be a complex of Rubisco and Rubisco activase. The purified enzyme activities from LaCl3-, CeCl3-treated spinach are 1.8 and 2.8 times that of the control, the intensities of absorption and fluorescence are significantly higher than that of the purified Rubisco from the control, and the total sulfhydryl groups and available sulfhydryl groups are 36-39-SH per mol enzyme, 14-25-SH per mol enzyme more than those of the purified Rubisco from the control, respectively. The CID spectra show that the secondary structure of the purified enzyme from LaCl3-, CeCl3 -treated spinach is very different from the control. The enzyme activities from LaCl3-, CeCl3-treated spinach in vivo are 1.5 and 1.9 times those of the control.
KeywordLa3+ Ce3+ spinach Rubisco complex of Rubisco and Rubisco acitivase spectral characteristics
Subject AreaChemistry
DOI10.1007/BF02990915
Indexed BySCI
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000228190500010
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/225897
Collection多学科研究中心
Recommended Citation
GB/T 7714
Hong, F,Liu, C,Zheng, L,et al. Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2005,48(1):#REF!.
APA Hong, F.,Liu, C.,Zheng, L.,Wang, XF.,Wu, K.,...&陶冶.(2005).Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach.SCIENCE IN CHINA SERIES B-CHEMISTRY,48(1),#REF!.
MLA Hong, F,et al."Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach".SCIENCE IN CHINA SERIES B-CHEMISTRY 48.1(2005):#REF!.
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