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Quantitative analysis of iron in protein bands existing in polyacrylamide gel matrix with synchrotron radiation x-ray fluorescence
其他题名聚丙烯酰胺凝胶基体中蛋白条带内金属含量的同步辐射X荧光定量分析
Liu YB(刘颖斌); Gao YX(高愈希); Chen CY(陈春英); Li B(李柏); Yu HW(喻宏伟); Wang JX(王江雪); He W(何伟); Huang YY(黄宇营); Chai ZF(柴之芳); Liu, YB; Gao, YX; Chen, CY; Li, B; Yu, HW; Wang, JX; He, W; Huang, YY; Chai, ZF
2005
发表期刊HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION
卷号29页码:#REF!
通讯作者Liu, YB (reprint author), CAS, Inst High Energy Phys, Beijing 100049, Peoples R China.
摘要A procedure has been proposed for quantification of Fe in protein bands in polyacrylamide gel matrix with synchrotron radiation X-ray fluorescence(SRXRF) analysis. Human hemoglobin was separated with isoelectric focusing (IEF), and Fe amounts in protein bands were measured with SRXRF. Calibration standards were prepared by electrophoresis of bovine serum albumin containing known amounts of Fe with stacking gel. The calibration curves can be obtained in a range of 0-5.1ng Fe and linear relationship between dosage of metals and fluorescent intensity can be observed (R-2 = 0.9897). The method provides detection limit of 0.63ng Fe, and the recovery of Fe determination is about 106%. The hyphenated technique of SRXRF and IEF has advantages of quantifying trace elements in proteins and identifying the proteins simultaneously. It can be employed for speciation analysis of trace elements in biological samples.
文章类型Article
关键词trace element speciation analysis synchrotron radiation X-ray fluorescence electrophoresis metalloprotein
学科领域Physics
收录类别SCI
WOS类目Physics, Nuclear ; Physics, Particles & Fields
WOS记录号WOS:000234206900016
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/225844
专题院士
实验物理中心
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GB/T 7714
Liu YB,Gao YX,Chen CY,et al. Quantitative analysis of iron in protein bands existing in polyacrylamide gel matrix with synchrotron radiation x-ray fluorescence[J]. HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,2005,29:#REF!.
APA 刘颖斌.,高愈希.,陈春英.,李柏.,喻宏伟.,...&Chai, ZF.(2005).Quantitative analysis of iron in protein bands existing in polyacrylamide gel matrix with synchrotron radiation x-ray fluorescence.HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,29,#REF!.
MLA 刘颖斌,et al."Quantitative analysis of iron in protein bands existing in polyacrylamide gel matrix with synchrotron radiation x-ray fluorescence".HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION 29(2005):#REF!.
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