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Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach
Hong, FS; Wang, XF; Liu, C; Su, GX; Song, WP; Wu, K; Tao, Y; Zhao, GW; Tao Y(陶冶)
Corresponding AuthorHong, FS (reprint author), Suzhou Univ, Coll Life Sci, Suzhou 215006, Peoples R China.
AbstractIt was studied by spectroscopy that PSII reaction center complex consisting of three polypeptides, D-1, D-2 and Cytb559, were purified from PSII particle of CeCl3 treated spinach. The results of the experiment show that Ce3+, could improve the growth of spinach, and accelerate electron transport of PSII particles. Of chl-a. of UIV-Vis spectrum of D1/D2/Cytb559 complex, Soret band was blue-shifted by 3 nm and Q band by 2 nm, respectively, and the fluorescence emission peak was blue-shifted by 5 nm in CeCl3-treated spinach compared with the one in control. By the extended X-ray absorption fine structure (EXAFS) spectroscopy methods, it has been found that Ce3+ is coordinated with 8 nitrogen atoms in the first coordination shell with Ce-N bond length of 0.253 nm, and Ce3+ with 6 oxygen atoms in the second coordination shell with Ce-O bond length of 0.32 nm. However, the secondary structure of D1/D2/Cytb559 complex by circular dichroism (CD) spectroscopy has no significant change after CeCl3 treated. It might be that Ce3+ binds to porphyrin rings of chlorophyll and oxygen of amino acid residue of polypeptide in D1/D2/Cytb559 complex, and then accelerates the primary reaction of PSII, intensifies function of P680(+) primary electron donor of Dl/D2/Cytb559, but there is little change in conformation of PSII reaction center complex.
KeywordCe3+ spinach D1/D2/Cytb559 complex photosynthesis spectra characteristic
Subject AreaChemistry
Indexed BySCI
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000181415100007
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Document Type期刊论文
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GB/T 7714
Hong, FS,Wang, XF,Liu, C,et al. Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2003,46(1):#REF!.
APA Hong, FS.,Wang, XF.,Liu, C.,Su, GX.,Song, WP.,...&陶冶.(2003).Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach.SCIENCE IN CHINA SERIES B-CHEMISTRY,46(1),#REF!.
MLA Hong, FS,et al."Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach".SCIENCE IN CHINA SERIES B-CHEMISTRY 46.1(2003):#REF!.
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