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XAS study of a venom metalloprotein
Li SJ(李树军); Hu TD(胡天斗); Xie YN(谢亚宁); Wu ZY(吴自玉); Li, SJ; Zhao, W; Chu, WS; Niu, LW; Teng, MK; Hu, TD; Xie, YN; Wu, ZY
Corresponding AuthorWu, ZY (reprint author), Chinese Acad Sci, Inst High Energy Phys, Beijing 100039, Peoples R China.
AbstractIn this paper, we probed the Acutolysin A-a Zinc protein purified from venom -by XAS technique, and its XAS curves in solution state of different pH values, were obtained. It was confirmed that the intense X-Rays didnt damage the samples during the experiment, and the curves among different pH values did present some differences. Through the comparison between simulated EXAFS curve based on PDB structure and an experimental signal, we concluded that the PDB data does not qualify for the fine structure research due to the limited resolution. At the same time, XANES analysis result could not reconstruct the experimental EXAFS curve either, because the PDB cluster used in the XANES calculation is free of Hydrogen atoms, therefore, a Hydrogen-contained cluster might be essential to the good fitting of XANES.
KeywordAeutolysin A metalloprotein zinc protein XANES EXAFS
Subject AreaPhysics
Indexed BySCI
WOS SubjectPhysics, Nuclear ; Physics, Particles & Fields
WOS IDWOS:000220592300005
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Document Type期刊论文
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GB/T 7714
Li SJ,Hu TD,Xie YN,et al. XAS study of a venom metalloprotein[J]. HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,2003,27:#REF!.
APA 李树军.,胡天斗.,谢亚宁.,吴自玉.,Li, SJ.,...&Wu, ZY.(2003).XAS study of a venom metalloprotein.HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,27,#REF!.
MLA 李树军,et al."XAS study of a venom metalloprotein".HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION 27(2003):#REF!.
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