Chen, CY (reprint author), Chinese Acad Sci, Inst High Energy Phys, POB 2732, Beijing 100080, Peoples R China.
Polysaccharides prepared from Chinese traditional medicine "Ruoye" (Indocalamus tesselatus), a kind of bamboo leaves, were studied. Eight crude polysaccharides were isolated from the dried leaves by successive extraction with 85% ethanol (FE), 0.9% sodium chloride (FS), hot water (FI), 1% ammonium oxalate (FII), 5% NaOH (FIII-a, FIII-b) and 20% NaOH (FIV-a, FIV-b). They were decolorized by hydrogen peroxide treatment and the protein was removed with Savag's method. The polysaccharides were precipitated by three volumes of absolute ethanol. After further purification by gel filtration chromatography on a Sephadex G-200 column, these polysaccharides were lyophilized. Their physicochemical properties and structural feature were elucidated by modern analytical technique such as UV, elemental analysis and high performance gel permeation chromatography. From the results of partial hydrolysis, FE and FIII-b have a main chain comprised solely of glucuronic acid and xylose residues, respectively. Copyright (C) 1999 John Wiley & Sons, Ltd.
Chen CY,Chen, CY,Ding, YQ,et al. Study on the isolation, purification and physicochemical properties of polysaccharides from Indocalamus tesselatus[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(1):11-14.
陈春英.,Chen, CY.,Ding, YQ.,Elmahadi, EA.,Zhou, JY.,...&Xu, HB.(1999).Study on the isolation, purification and physicochemical properties of polysaccharides from Indocalamus tesselatus.BIOMEDICAL CHROMATOGRAPHY,13(1),11-14.
陈春英,et al."Study on the isolation, purification and physicochemical properties of polysaccharides from Indocalamus tesselatus".BIOMEDICAL CHROMATOGRAPHY 13.1(1999):11-14.