Study on the isolation, purification and physicochemical properties of polysaccharides from Indocalamus tesselatus
Chen CY(陈春英); Chen, CY; Ding, YQ; Elmahadi, EA; Zhou, JY; Li, Y; Xu, HB
刊名BIOMEDICAL CHROMATOGRAPHY
1999
卷号13期号:1页码:11-14
学科分类Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy
DOI10.1002/(SICI)1099-0801(199902)13:1<11::AID-BMC764>3.0.CO;2-V
通讯作者Chen, CY (reprint author), Chinese Acad Sci, Inst High Energy Phys, POB 2732, Beijing 100080, Peoples R China.
文章类型Article
英文摘要Polysaccharides prepared from Chinese traditional medicine "Ruoye" (Indocalamus tesselatus), a kind of bamboo leaves, were studied. Eight crude polysaccharides were isolated from the dried leaves by successive extraction with 85% ethanol (FE), 0.9% sodium chloride (FS), hot water (FI), 1% ammonium oxalate (FII), 5% NaOH (FIII-a, FIII-b) and 20% NaOH (FIV-a, FIV-b). They were decolorized by hydrogen peroxide treatment and the protein was removed with Savag's method. The polysaccharides were precipitated by three volumes of absolute ethanol. After further purification by gel filtration chromatography on a Sephadex G-200 column, these polysaccharides were lyophilized. Their physicochemical properties and structural feature were elucidated by modern analytical technique such as UV, elemental analysis and high performance gel permeation chromatography. From the results of partial hydrolysis, FE and FIII-b have a main chain comprised solely of glucuronic acid and xylose residues, respectively. Copyright (C) 1999 John Wiley & Sons, Ltd.
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
收录类别SCI
WOS记录号WOS:000078518500002
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文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/225483
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
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GB/T 7714
Chen CY,Chen, CY,Ding, YQ,et al. Study on the isolation, purification and physicochemical properties of polysaccharides from Indocalamus tesselatus[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(1):11-14.
APA 陈春英.,Chen, CY.,Ding, YQ.,Elmahadi, EA.,Zhou, JY.,...&Xu, HB.(1999).Study on the isolation, purification and physicochemical properties of polysaccharides from Indocalamus tesselatus.BIOMEDICAL CHROMATOGRAPHY,13(1),11-14.
MLA 陈春英,et al."Study on the isolation, purification and physicochemical properties of polysaccharides from Indocalamus tesselatus".BIOMEDICAL CHROMATOGRAPHY 13.1(1999):11-14.
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