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Tyrosine phosphorylation/dephosphorylation regulates peroxynitrite-mediated peptide nitration
Shi WQ(石伟群); Xu DD(徐殿斗); Shi, WQ; Cai, H; Xu, DD; Su, XY; Lei, P; Zhao, YF; Li, YM
Corresponding AuthorLi, YM (reprint author), Tsing Hua Univ, Dept Chem, Key Lab Bioorgan Phosphorous Chem, Beijing 100084, Peoples R China.
AbstractProteins are targets of reactive nitrogen species such as peroxynitrite and nitrogen dioxide. Among the various amino acids in proteins, tyrosine and tryptophan residues are especially susceptible to attack by reactive nitrogen species. On the other hand, protein tyrosine phosphorylation has gained much attention in respect to cellular regulatory events and signal transduction. Peroxynitrite-mediated nitration of peptide YPPPPPW and phosphopeptide pYPPPPPW were studied at pH 7.4. The predominant nitrated products were separated and identified by reverse phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry (LC-MS). The nitration sites were established by tandem electrospray ionization-mass spectrometry (LC-MS/MS). A regulatory effect of tyrosine phosphorylation/dephosphorylation on peptide nitration was observed. YPPPPPW was predominantly nitrated at tyrosine residue while pYPPPPPW was nitrated at tryptophan one. Our results can help in understanding the biochemical significance of the relationship of tyrosine phosphorylation and nitration in proteins. (c) 2007 Elsevier B.V All rights reserved.
Keywordtyrosine tryptophan nitration phosphorylation peroxynitrite
Subject AreaEndocrinology & Metabolism; Physiology
Indexed BySCI
WOS SubjectEndocrinology & Metabolism ; Physiology
WOS IDWOS:000251601800001
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Document Type期刊论文
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GB/T 7714
Shi WQ,Xu DD,Shi, WQ,et al. Tyrosine phosphorylation/dephosphorylation regulates peroxynitrite-mediated peptide nitration[J]. REGULATORY PEPTIDES,2007,144(1-3):1-5.
APA 石伟群.,徐殿斗.,Shi, WQ.,Cai, H.,Xu, DD.,...&Li, YM.(2007).Tyrosine phosphorylation/dephosphorylation regulates peroxynitrite-mediated peptide nitration.REGULATORY PEPTIDES,144(1-3),1-5.
MLA 石伟群,et al."Tyrosine phosphorylation/dephosphorylation regulates peroxynitrite-mediated peptide nitration".REGULATORY PEPTIDES 144.1-3(2007):1-5.
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