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Size-dependent impact of CNTs on dynamic properties of calmodulin
Gao J(高剑); Gao, J; Wang, LM; Kang, SG; Zhao, LN; Ji, MJ; Chen, CY; Zhao, YL; Zhou, RH; Li, JY;; Zhao LN(赵丽娜); Zhao YL(赵宇亮); Li JY(李敬源)
2014
发表期刊NANOSCALE
卷号6期号:21页码:12828-12837
摘要There are growing concerns about the biosafety of nanomaterials such as carbon nanotubes (CNTs) as their applications become more widespread. We report here a theoretical and experimental study of the binding of various sizes of CNTs [CNT (4,4), (5,5), (6,6) and (7,7)] to calmodulin (CaM) protein and, in particular, their impact on the Ca2+-dependent dynamic properties of CaM. Our simulations show that all the CNTs can plug into the hydrophobic binding pocket of Ca2+-bound CaM with binding affinities comparable with the native substrate M13 peptide. Even though CNT (4,4) shows a similar behavior to the M13 peptide in its dissociation from Ca2+-free CaM, wider CNTs still bind firmly to CaM, indicating a potential failure of Ca2+ regulation. Such a size-dependent impact of CNTs on the dynamic properties of CaM is a result of the excessively strong hydrophobic interactions between the wider CNTs and CaM. These simulation results were confirmed by circular dichroism spectroscopy, which showed that the secondary structures of CaM become insensitive to Ca2+ concentrations after the addition of CNTs. Our findings indicate that the cytotoxicity of nanoparticles to proteins arises not only from the inhibition of static protein structures (binding pockets), but also from impacts on their dynamic properties.
学科领域Chemistry; Science & Technology - Other Topics; Materials Science; Physics
DOI10.1039/c4nr01623h
收录类别SCI
WOS记录号WOS:000344997500076
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/225200
专题多学科研究中心
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GB/T 7714
Gao J,Gao, J,Wang, LM,et al. Size-dependent impact of CNTs on dynamic properties of calmodulin[J]. NANOSCALE,2014,6(21):12828-12837.
APA 高剑.,Gao, J.,Wang, LM.,Kang, SG.,Zhao, LN.,...&李敬源.(2014).Size-dependent impact of CNTs on dynamic properties of calmodulin.NANOSCALE,6(21),12828-12837.
MLA 高剑,et al."Size-dependent impact of CNTs on dynamic properties of calmodulin".NANOSCALE 6.21(2014):12828-12837.
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