A yeast bioassay for direct measurement of thyroid hormone disrupting effects in water without sample extraction, concentration, or sterilization
Li, J; Ren, SJ; Han, SL; Li, N;李娜(多)
刊名CHEMOSPHERE
2014
卷号100页码:139-145
关键词In vitro bioassay Thyroid receptor Environment water sample Thyroid disrupting chemicals
学科分类Environmental Sciences & Ecology
DOI10.1016/j.chemosphere.2013.11.054
英文摘要The present study introduces an improved yeast bioassay for rapid yet sensitive evaluation of thyroid hormone disruption at the level of thyroid receptor (TR) in environmental water samples. This assay does not require water sample preparation and thus requires very little hands-on time. Based on different beta-galactosidase substrates, two modified bioassays, a colorimetric bioassay and a chemiluminescent bioassay, were developed. The compounds tested included the known thyroid hormone 3,3',5-triiodo-L-thyronine (T-3), the specific TR antagonist amiodarone hydrochloride (AH) and phthalate esters (PAEs), which potentially disrupt thyroid hormone signaling. The EC50 values for T-3 were similar to those previously obtained using a 96-well plate bioassay. TR antagonism by AH was studied in the presence of 2.5 x 10(-7) M Ts, and the concentration producing 20% of the maximum effect (RIC20) for AH was 3.1 x 10(-7) M and 7.8 x 10(-9) M for the colorimetric bioassay and chemiluminescent bioassay, respectively. None of the tested PAEs induced B-galactosidase expression, but diethylhexyl phthalate, benzyl butyl phthalate and dibutyl phthalate demonstrated TR antagonism. Furthermore, water samples collected from Guanting reservoir in Beijing were evaluated. Although TR agonism was not observed, antagonism was detected in all water samples and is expressed as AH equivalents. The toxicology equivalent quantity values obtained by the chemiluminescent bioassay ranged from 21.2 +/- 1.6 to 313.9 +/- 28.8 mu g L-1 AH, and similar values were obtained for the colorimetric bioassay. The present study shows that the modified yeast bioassay can be used as a valuable tool for quantification of thyroid hormone disrupting effects in environmental water samples. (C) 2013 Elsevier Ltd. All rights reserved.
收录类别SCI
WOS记录号WOS:000332264800020
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224890
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
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GB/T 7714
Li, J,Ren, SJ,Han, SL,et al. A yeast bioassay for direct measurement of thyroid hormone disrupting effects in water without sample extraction, concentration, or sterilization[J]. CHEMOSPHERE,2014,100:139-145.
APA Li, J,Ren, SJ,Han, SL,&Li, N;李娜.(2014).A yeast bioassay for direct measurement of thyroid hormone disrupting effects in water without sample extraction, concentration, or sterilization.CHEMOSPHERE,100,139-145.
MLA Li, J,et al."A yeast bioassay for direct measurement of thyroid hormone disrupting effects in water without sample extraction, concentration, or sterilization".CHEMOSPHERE 100(2014):139-145.
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