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Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets
Zhou, T; Zhang, B; Wei, P; Du, YP; Zhou, HJ; Yu, MF; Yan, L; Zhang, WD; Nie, GJ; Chen, CY; Tu, YP; Wei, TT;晏亮; Chen CY(陈春英)
2014
发表期刊BIOMATERIALS
卷号35期号:37页码:9833-9843
摘要Recent advances in nanomedicine provide promising alternatives for cancer treatment that may improve the survival of patients with metastatic disease. The goal of the present study was to evaluate graphene oxide (GO) as a potential anti-metastatic agent. For this purpose, GO was modified with polyethylene glycol (PEG) to form PEG-modified GO (PEG-GO), which improves its aqueous stability and biocompatibility. We show here that PEG-GO exhibited no apparent effects on the viability of breast cancer cells (MDA-MB-231, MDA-MB-436, and SK-BR-3) or non-cancerous cells (MCF-10A), but inhibited cancer cell migration in vitro and in vivo. Analysis of cellular energy metabolism revealed that PEG-GO significantly impaired mitochondrial oxidative phosphorylation (OXPHOS) in breast cancer cells; however, PEG-GO showed no effect on OXPHOS in non-cancerous cells. To explore the underlying mechanisms, a SILAC (Stable Isotope Labeling by Amino acids in Cell culture) labeling strategy was used to quantify protein expression in PEG-GO-exposed breast cancer versus non-cancerous cells. The results indicated that PEG-GO selectively down-regulated PGC-1 alpha in breast cancer cells and thus modified the expression of diverse energy generation-related proteins, which accounts for the inhibition of OXPHOS. The inhibition of OXPHOS by PEG-GO significantly reduced ATP production and impaired assembly of the F-actin cytoskeleton in breast cancer cells, which is required for the migratory and invasive phenotype of cancer cells. Taken together, these effects of PEG-GO on cancer cell metastasis may allow the development of a new approach to treat metastatic breast cancer. (C) 2014 Elsevier Ltd. All rights reserved.
关键词PEG-modified graphene oxide Breast cancer cell Metastasis Cytoskeletal assembly Mitochondrial oxidative phosphorylation
学科领域Engineering; Materials Science
DOI10.1016/j.biomaterials.2014.08.033
收录类别SCI
WOS记录号WOS:000343639700009
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224863
专题多学科研究中心
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GB/T 7714
Zhou, T,Zhang, B,Wei, P,et al. Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets[J]. BIOMATERIALS,2014,35(37):9833-9843.
APA Zhou, T.,Zhang, B.,Wei, P.,Du, YP.,Zhou, HJ.,...&陈春英.(2014).Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets.BIOMATERIALS,35(37),9833-9843.
MLA Zhou, T,et al."Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets".BIOMATERIALS 35.37(2014):9833-9843.
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