IHEP OpenIR
Fates of Fe3O4 and Fe3O4@SiO2 nanoparticles in human mesenchymal stem cells assessed by synchrotron radiation-based techniques
Tian, F; Chen, GC; Yi, PW; Zhang, JC; Li, AG; Zhang, J; Zheng, LR; Deng, ZW; Shi, Q; Peng, R; Wang, QB;张静; Zheng LR(郑黎荣)
2014
Source PublicationBIOMATERIALS
ISSN0142-9612
EISSN1878-5905
Volume35Issue:24Pages:6412-6421
AbstractSuperparamagnetic iron oxide nanoparticles (SPIOs) have been widely used as the magnetic resonance imaging (MRI) contrast agent in biomedical studies and clinical applications, with special interest recently in in vivo stem cell tracking. However, a full understanding of the fate of SPIOs in cells has not been achieved yet, which is particularly important for stem cells since any change of the microenvironment may disturb their propagation and differentiation behaviors. Herein, synchrotron radiation-based X-ray fluorescence (XRF) in combination with X-ray absorption spectroscopy (XAS) were used to in situ reveal the fate of Fe3O4 and Fe3O4@SiO2 NPs in human mesenchymal stem cells (hMSCs), in which the dynamic changes of their distribution and chemical speciation were precisely determined. The XAS analysis evidences that Fe3O4 NPs cultured with hMSCs are quite stable and almost keep their initial chemical form up to 14 days, which is contradictory to the previous report that Fe3O4 NPs were unstable in cell labeling assessed by using a simplified lysosomal model system. Coating with a SiO2 shell, Fe3O4@SiO2 NPs present higher stability in hMSCs without detectable changes of their chemical form. In addition, XRF analysis demonstrates that Fe3O4@SiO2 NPs can label hMSCs in a high efficiency manner and are solely distributed in cytoplasm during cell proliferation, making it an ideal probe for in vivo stem cell tracking. These findings with the help of synchrotron radiation-based XAS and XRF improve our understanding of the fate of SPIOs administered to hMSCs and will help the future design of SPIOs for safe and efficient stem cells tracking. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordFe3O4 nanoparticles Fe3O4@SiO2 nanoparticles Synchrotron radiation Human mesenchymal stem cells Distribution Chemical speciation
Subject AreaEngineering; Materials Science
DOI10.1016/j.biomaterials.2014.04.052
Indexed BySCI
Language英语
WOS IDWOS:000338804500026
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/224860
Collection中国科学院高能物理研究所
Recommended Citation
GB/T 7714
Tian, F,Chen, GC,Yi, PW,et al. Fates of Fe3O4 and Fe3O4@SiO2 nanoparticles in human mesenchymal stem cells assessed by synchrotron radiation-based techniques[J]. BIOMATERIALS,2014,35(24):6412-6421.
APA Tian, F.,Chen, GC.,Yi, PW.,Zhang, JC.,Li, AG.,...&郑黎荣.(2014).Fates of Fe3O4 and Fe3O4@SiO2 nanoparticles in human mesenchymal stem cells assessed by synchrotron radiation-based techniques.BIOMATERIALS,35(24),6412-6421.
MLA Tian, F,et al."Fates of Fe3O4 and Fe3O4@SiO2 nanoparticles in human mesenchymal stem cells assessed by synchrotron radiation-based techniques".BIOMATERIALS 35.24(2014):6412-6421.
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