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Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry
Wang M(王萌); Zheng LN(郑丽娜); Wang B(汪冰); Wang, M; Zheng, LN; Wang, B; Chen, HQ; Zhao, YL; Chai, ZF; Reid, HJ; Sharp, BL; Feng, WY; Chen HQ(陈汉卿); Zhao YL(赵宇亮); Chai ZF(柴之芳); Feng WY(丰伟悦)
2014
发表期刊ANALYTICAL CHEMISTRY
卷号86期号:20页码:10252-10256
摘要Single cell analysis has become an important field of research in recent years reflecting the heterogeneity of cellular responses in biological systems. Here, we demonstrate a new method, based on laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS), which can quantify in situ gold nanoparticles (Au NPs) in single cells. Dried residues of picoliter droplets ejected by a commercial inkjet printer were used to simulate matrix-matched calibration standards. The gold mass in single cells exposed to 100 nM NIST Au NPs (Reference material 8012, 30 nm) for 4 h showed a log-normal distribution, ranging from 1.7 to 72 fg Au per cell, which approximately corresponds to 9 to 370 Au NPs per cell. The average result from 70 single cells (15 +/- 13 fg Au per cell) was in good agreement with the result from an aqua regia digest solution of 1.2 x 10(6) cells (18 +/- 1 fg Au per cell). The limit of quantification was 1.7 fg Au. This paper demonstrates the great potential of LA-ICPMS for single cell analysis and the beneficial study of biological responses to metal drugs or NPs at the single cell level.
学科领域Chemistry
DOI10.1021/ac502438n
收录类别SCI
WOS记录号WOS:000343639800037
引用统计
被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224810
专题院士
多学科研究中心
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Wang M,Zheng LN,Wang B,et al. Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2014,86(20):10252-10256.
APA 王萌.,郑丽娜.,汪冰.,Wang, M.,Zheng, LN.,...&丰伟悦.(2014).Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry.ANALYTICAL CHEMISTRY,86(20),10252-10256.
MLA 王萌,et al."Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry".ANALYTICAL CHEMISTRY 86.20(2014):10252-10256.
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