Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes
Liu R(刘茹); Wang YL(王亚玲); Liu, R; Liu, L; Liang, J; Wang, YL; Wei, YT; Gao, FP; Gao, L; Gao, XY; Wei YT(魏岳腾); Gao FP(高福平); Gao J(高靓); Gao XY(高学云)
刊名ANALYTICAL CHEMISTRY
2014
卷号86期号:6页码:3048-3052
学科分类Chemistry
DOI10.1021/ac500499q
英文摘要Myocardial ischemia is featured by a significant increase in the cytoplasm proton concentration, and such a proton change may be applied as an index for earlier ischemic heart disease diagnostics. But such a pH change in a live heart cell is difficult to monitor as a normal fluorescent probe cannot specifically transport into the cytoplasm of an ischemic cell. This is because the heart cell contains condensed myofibrils which are tight barriers for a normal probe to penetrate. We design fluorescent probes, single-strand DNA wrapped single-wall carbon nanotubes (ssDNA-SWCNTs), where the ssDNA is labeled by the dye molecule hexachloro-6-carboxyfluorescein (HEX). This nanoprobe could transport well into a live heart cell and locate in the cytoplasm to sensitively detect the intracellular pH change of myocardial ischemia. Briefly, protons neutralize the negative charges of nanoprobes in the cytoplasm. This will weaken the stability of nanoprobes and further tune their aggregation. Such aggregations induce the HEX of some nanoprobes condensed together and further result in their fluorescence quenching. The nanoprobes are advantaged in penetrating condensed myofibrils of the heart cell, and their fluorescence intensity is sensitive to the proton concentration change in the live cell cytoplasm. This new method may provide great assistance in earlier cardiopathy diagnosis in the future.
收录类别SCI
WOS记录号WOS:000333235700032
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224809
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
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Liu R,Wang YL,Liu, R,et al. Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes[J]. ANALYTICAL CHEMISTRY,2014,86(6):3048-3052.
APA 刘茹.,王亚玲.,Liu, R.,Liu, L.,Liang, J.,...&高学云.(2014).Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes.ANALYTICAL CHEMISTRY,86(6),3048-3052.
MLA 刘茹,et al."Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes".ANALYTICAL CHEMISTRY 86.6(2014):3048-3052.
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