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Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa
Chen Z(陈真); Gao ZQ(高增强); Xu JH(徐建华); Zhang H(张衡); 董宇辉;Chen, Z; Gao, ZQ; Hu, HD; Xu, JH; Zhang, H; Dong, YH
2014
发表期刊ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
卷号70期号:7页码:903-905
摘要The putative protein PA5089 from Pseudomonas aeruginosa has recently been identified as a Tle5 phospholipase effector from a type VI secretion system (T6SS), and its toxicity can be neutralized by the cognate immunity protein Tli5 (PA5088). Here, the expression, purification, crystallization and preliminary crystallographic analysis of PA5088 are reported. X-ray diffraction data were collected from selenomethionine-derivatized PA5088 crystals to a resolution of 2.55 angstrom. The crystals belonged to space group P2(1), with unit-cell parameters a = 64.002, b = 104.744, c = 90.168 angstrom.
学科领域Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S2053230X14010164
收录类别SCI
WOS记录号WOS:000338923200010
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224795
专题多学科研究中心
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Chen Z,Gao ZQ,Xu JH,et al. Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2014,70(7):903-905.
APA 陈真.,高增强.,徐建华.,张衡.,董宇辉;Chen, Z.,...&Dong, YH.(2014).Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,70(7),903-905.
MLA 陈真,et al."Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 70.7(2014):903-905.
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