The bacterial type VI secretion system (T6SS), a dynamic organelle, participates in microbial competition by transporting toxic effector molecules to neighbouring cells to kill competitors. TsiV3, a recently defined T6SS immunity protein in Vibrio cholerae, possesses self-protection against killing by T6SS predatory cells by directly binding to and inhibiting their effector protein VgrG-3. Structural information about TsiV3 could help to illuminate its specific mechanism. In this study, TsiV3 from V. cholerae was cloned, expressed and crystallized and single-crystal X-ray diffraction data sets were collected to a resolution of 2.55 angstrom. Specifically, the crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 73.3, b = 78.12, c = 106.18 angstrom. Matthews coefficient calculations indicated that the crystal may contain six TsiV3 molecules in one asymmetric unit, with a V-M value of 2.25 angstrom(3) Da(-1) and a solvent content of 45.42%.
Zhang, JL,Zhang, H,Liu Y,et al. Crystallization and preliminary X-ray study of TsiV3 from Vibrio cholerae[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2014,70(3):335-338.
Zhang, JL.,Zhang, H.,刘颖.,Liu, Y.,Zhan, LH.,...&董宇辉.(2014).Crystallization and preliminary X-ray study of TsiV3 from Vibrio cholerae.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,70(3),335-338.
Zhang, JL,et al."Crystallization and preliminary X-ray study of TsiV3 from Vibrio cholerae".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 70.3(2014):335-338.