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Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting
Hu, HD; Zhang, H; Gao, ZQ; Wang, DQ; Liu, GF; Xu, JH; Lan, K; Dong, YH;胡海岱; Zhang H(张衡); Gao ZQ(高增强); Liu GF(刘广峰); Xu JH(徐建华); Dong YH(董宇辉); Wang DQ(王东琪)
2014
发表期刊ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
卷号70期号:8页码:2175-2185
摘要A diverse superfamily of phospholipases consisting of the type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have recently been identified as antibacterial effectors that hydrolyze membrane phospholipids. These effectors show no significant homology to known lipases, and their mechanism of membrane targeting and hydrolysis of phospholipids remains unknown. Here, the crystal structure of Tle1 (similar to 96.5 kDa) from Pseudomonas aeruginosa refined to 2.0 angstrom resolution is reported, representing the first structure of this superfamily. Its overall structure can be divided into two distinct parts, the phospholipase catalytic module and the putative membrane-anchoring module; this arrangement has not previously been observed in known lipase structures. The phospholipase catalytic module has a canonical alpha/beta-hydrolase fold and mutation of any residue in the Ser-Asp-His catalytic triad abolishes its toxicity. The putative membrane-anchoring module adopts an open conformation composed of three amphipathic domains, and its partial folds are similar to those of several periplasmic or membrane proteins. A cell-toxicity assay revealed that the putative membrane-anchoring module is critical to Tle1 antibacterial activity. A molecular-dynamics (MD) simulation system in which the putative membrane-anchoring module embedded into a bilayer was stable over 50 ns. These structure-function studies provide insight into the hydrolysis and membrane-targeting process of the unique phospholipase Tle1.
学科领域Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S1399004714012899
收录类别SCI
WOS记录号WOS:000340246000011
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224793
专题多学科研究中心
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GB/T 7714
Hu, HD,Zhang, H,Gao, ZQ,et al. Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2014,70(8):2175-2185.
APA Hu, HD.,Zhang, H.,Gao, ZQ.,Wang, DQ.,Liu, GF.,...&王东琪.(2014).Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,70(8),2175-2185.
MLA Hu, HD,et al."Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 70.8(2014):2175-2185.
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