IHEP OpenIR  > 多学科研究中心
Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase
Zhu, CF; Gao, WY; Zhao, K; Qin, XH; Zhang, YJ; Peng, X; Zhang, L; Dong, YH; Zhang, WY; Li, P; Wei, W; Gong, Y; Yu, XF;董宇辉
2013
发表期刊NATURE COMMUNICATIONS
卷号4页码:2722
摘要SAMHD1 is a dGTP-activated deoxynucleoside triphosphate triphosphohydrolase (dNTPase) whose dNTPase activity has been linked to HIV/SIV restriction. The mechanism of its dGTP-activated dNTPase function remains unclear. Recent data also indicate that SAMHD1 regulates retrotransposition of LINE-1 elements. Here we report the 1.8-angstrom crystal structure of homotetrameric SAMHD1 in complex with the allosteric activator and substrate dGTP/dATP. The structure indicates the mechanism of dGTP-dependent tetramer formation, which requires the cooperation of three subunits and two dGTP/dATP molecules at each allosteric site. Allosteric dGTP binding induces conformational changes at the active site, allowing a more stable interaction with the substrate and explaining the dGTP-induced SAMHD1 dNTPase activity. Mutations of dGTP binding residues in the allosteric site affect tetramer formation, dNTPase activity and HIV-1 restriction. dGTP-triggered tetramer formation is also important for SAMHD1-mediated LINE-1 regulation. The structural and functional information provided here should facilitate future investigation of SAMHD1 function, including dNTPase activity, LINE-1 modulation and HIV-1 restriction.
学科领域Science & Technology - Other Topics
DOI10.1038/ncomms3722
收录类别SCI
WOS记录号WOS:000328021600003
引用统计
被引频次:51[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224467
专题多学科研究中心
推荐引用方式
GB/T 7714
Zhu, CF,Gao, WY,Zhao, K,et al. Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase[J]. NATURE COMMUNICATIONS,2013,4:2722.
APA Zhu, CF.,Gao, WY.,Zhao, K.,Qin, XH.,Zhang, YJ.,...&Yu, XF;董宇辉.(2013).Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase.NATURE COMMUNICATIONS,4,2722.
MLA Zhu, CF,et al."Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase".NATURE COMMUNICATIONS 4(2013):2722.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
8450.pdf(1793KB)期刊论文作者接受稿限制开放CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhu, CF]的文章
[Gao, WY]的文章
[Zhao, K]的文章
百度学术
百度学术中相似的文章
[Zhu, CF]的文章
[Gao, WY]的文章
[Zhao, K]的文章
必应学术
必应学术中相似的文章
[Zhu, CF]的文章
[Gao, WY]的文章
[Zhao, K]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。