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Solution Small Angle X-ray Scattering (SAXS) Studies of RecQ from Deinococcus radiodurans and Its Complexes with Junction DNA Substrates
Wang WJ(王文佳); Hu HF(胡海峰); Wang, WJ; Hou, HF; Du, Q; Zhang, W; Liu, GF; Shtykova, EV; Xu, JH; Liu, P; Dong, YH; Liu GF(刘广峰); Xu JH(徐建华); Dong YH(董宇辉)
2013
发表期刊JOURNAL OF BIOLOGICAL CHEMISTRY
卷号288期号:45页码:32414-32423
摘要RecQ helicases, essential enzymes for maintaining genome integrity, possess the capability to participate in a wide variety of DNA metabolisms. They can initiate the homologous recombination repair pathway by unwinding damaged dsDNA and suppress hyper-recombination by promoting Holliday junction (HJ) migration. To learn how DrRecQ participates in the homologous recombination repair pathway, solution structures of Deinococcus radiodurans RecQ (DrRecQ) and its complexes with DNA substrates were investigated by small angle x-ray scattering. We found that the catalytic core and the most N-terminal HRDC (helicase and RNase D C-terminal) domain (HRDC1) undergo a conformational change to a compact state upon binding to a junction DNA. Furthermore, models of DrRecQ in complexes with two kinds of junction DNA (fork junction and HJ) were built based on the small angle x-ray scattering data, and together with the EMSA results, possible binding sites were proposed. It is demonstrated that two DrRecQ molecules bind to the opposite arms of HJ. This architecture is similar to the RuvAB complex and is hypothesized to be highly conserved in the other HJ migration proteins. This work provides us new clues to understand the roles DrRecQ plays in the RecFOR pathway.
关键词DNA Helicase Homologous Recombination Junctions Molecular Modeling X-ray Scattering RecQ SAXS
学科领域Biochemistry & Molecular Biology
DOI10.1074/jbc.M113.502112
收录类别SCI
WOS记录号WOS:000328681700026
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224366
专题多学科研究中心
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GB/T 7714
Wang WJ,Hu HF,Wang, WJ,et al. Solution Small Angle X-ray Scattering (SAXS) Studies of RecQ from Deinococcus radiodurans and Its Complexes with Junction DNA Substrates[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(45):32414-32423.
APA 王文佳.,胡海峰.,Wang, WJ.,Hou, HF.,Du, Q.,...&董宇辉.(2013).Solution Small Angle X-ray Scattering (SAXS) Studies of RecQ from Deinococcus radiodurans and Its Complexes with Junction DNA Substrates.JOURNAL OF BIOLOGICAL CHEMISTRY,288(45),32414-32423.
MLA 王文佳,et al."Solution Small Angle X-ray Scattering (SAXS) Studies of RecQ from Deinococcus radiodurans and Its Complexes with Junction DNA Substrates".JOURNAL OF BIOLOGICAL CHEMISTRY 288.45(2013):32414-32423.
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