Structural Basis for Interaction between Mycobacterium smegmatis Ms6564, a TetR Family Master Regulator, and Its Target DNA
Yang, SF; Gao, ZQ; Li, TT; Yang, M; Zhang, TY; Dong, YH; He, ZG;董宇辉
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2013
卷号288期号:33页码:23687-23695
学科分类Biochemistry & Molecular Biology
DOI10.1074/jbc.M113.468694
英文摘要Master regulators, which broadly affect expression of diverse genes, play critical roles in bacterial growth and environmental adaptation. However, the underlying mechanism by which such regulators interact with their cognate DNA remains to be elucidated. In this study, we solved the crystal structure of a broad regulator Ms6564 in Mycobacterium smegmatis and its protein-operator complex at resolutions of 1.9 and 2.5 angstrom, respectively. Similar to other typical TetR family regulators, two dimeric Ms6564 molecules were found to bind to opposite sides of target DNA. However, the recognition helix of Ms6564 inserted only slightly into the DNA major groove. Unexpectedly, 11 disordered water molecules bridged the interface of TetR family regulator DNA. Although the DNA was deformed upon Ms6564 binding, it still retained the conformation of B-form DNA. Within the DNA-binding domain of Ms6564, only two amino acids residues directly interacted with the bases of cognate DNA. Lys-47 was found to be essential for the specific DNA binding ability of Ms6564. These data indicate that Ms6564 can bind DNA with strong affinity but makes flexible contacts with DNA. Our study suggests that Ms6564 might slide more easily along the genomic DNA and extensively regulate the expression of diverse genes in M. smegmatis.
收录类别SCI
WOS记录号WOS:000330611400009
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224364
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
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Yang, SF,Gao, ZQ,Li, TT,et al. Structural Basis for Interaction between Mycobacterium smegmatis Ms6564, a TetR Family Master Regulator, and Its Target DNA[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(33):23687-23695.
APA Yang, SF.,Gao, ZQ.,Li, TT.,Yang, M.,Zhang, TY.,...&He, ZG;董宇辉.(2013).Structural Basis for Interaction between Mycobacterium smegmatis Ms6564, a TetR Family Master Regulator, and Its Target DNA.JOURNAL OF BIOLOGICAL CHEMISTRY,288(33),23687-23695.
MLA Yang, SF,et al."Structural Basis for Interaction between Mycobacterium smegmatis Ms6564, a TetR Family Master Regulator, and Its Target DNA".JOURNAL OF BIOLOGICAL CHEMISTRY 288.33(2013):23687-23695.
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