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RNase non-sensitive and endocytosis independent siRNA delivery system: delivery of siRNA into tumor cells and high efficiency induction of apoptosis
Jiang, XL; Wang, GB; Liu R(刘茹); Wang YL(王亚玲); 高学云;Liu, R; Wang, YL; Wang, YK; Qiu, XZ; Gao, XY
2013
发表期刊NANOSCALE
卷号5期号:16页码:7256-7264
摘要To date, RNase degradation and endosome/lysosome trapping are still serious problems for siRNA-based molecular therapy, although different kinds of delivery formulations have been tried. In this report, a cell penetrating peptide (CPP, including a positively charged segment, a linear segment, and a hydrophobic segment) and a single wall carbon nanotube (SWCNT) are applied together by a simple method to act as a siRNA delivery system. The siRNAs first form a complex with the positively charged segment of CPP via electrostatic forces, and the siRNA-CPP further coats the surface of the SWCNT via hydrophobic interactions. This siRNA delivery system is non-sensitive to RNase and can avoid endosome/lysosome trapping in vitro. When this siRNA delivery system is studied in Hela cells, siRNA uptake was observed in 98% Hela cells, and over 70% mRNA of mammalian target of rapamycin (mTOR) is knocked down, triggering cell apoptosis on a significant scale. Our siRNA delivery system is easy to handle and benign to cultured cells, providing a very efficient approach for the delivery of siRNA into the cell cytosol and cleaving the target mRNA therein.
学科领域Chemistry; Science & Technology - Other Topics; Materials Science; Physics
DOI10.1039/c3nr01183f
收录类别SCI
WOS记录号WOS:000322315600020
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224294
专题多学科研究中心
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Jiang, XL,Wang, GB,Liu R,et al. RNase non-sensitive and endocytosis independent siRNA delivery system: delivery of siRNA into tumor cells and high efficiency induction of apoptosis[J]. NANOSCALE,2013,5(16):7256-7264.
APA Jiang, XL.,Wang, GB.,刘茹.,王亚玲.,高学云;Liu, R.,...&Gao, XY.(2013).RNase non-sensitive and endocytosis independent siRNA delivery system: delivery of siRNA into tumor cells and high efficiency induction of apoptosis.NANOSCALE,5(16),7256-7264.
MLA Jiang, XL,et al."RNase non-sensitive and endocytosis independent siRNA delivery system: delivery of siRNA into tumor cells and high efficiency induction of apoptosis".NANOSCALE 5.16(2013):7256-7264.
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