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Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels
Wei YT(魏月腾); Liu R(刘茹); Wang YL(王亚玲); Wei, YT; Liu, R; Wang, YL; Zhao, YL; Cai, ZF; Gao, XY; Zhao YL(赵宇亮); Chai ZF(柴之芳); Gao XY(高学云)
2013
发表期刊ANALYST
卷号138期号:8页码:2302-2307
摘要A terbium based fluorescent probe was synthesized by coordinating terbium ions with a designed oligonucleotides (5'-ATATGGGGGATAT-3', termed GH5). GH5 improved the fluorescence of terbium ions by four orders of magnitude. The fluorescence enhancement of terbium ions by different oligonucleotides sequences indicated that the polyguanine loop of the hairpin GH5 is key to enhance terbium ion emission. The quantum yield of Tb-GH5 probe was 10.5% and the probe was photo-stable. The result of conductivity titration indicated that the stoichiometry of the probe is 3.5 Tb: 1 GH5, which is confirmed by fluorescence titration. This probe had high sensitivity and specificity for the detection of lead ions. The fluorescence intensity of this probe was linear with respect to lead concentration over a range 0.3-2.1 nM (R-2 = 0.99). The limit of detection for lead ions was 0.1 nM at a signal-to-noise ratio of 3.
学科领域Chemistry
DOI10.1039/c3an36795a
收录类别SCI
WOS记录号WOS:000316222300012
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224198
专题院士
多学科研究中心
推荐引用方式
GB/T 7714
Wei YT,Liu R,Wang YL,et al. Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels[J]. ANALYST,2013,138(8):2302-2307.
APA 魏月腾.,刘茹.,王亚玲.,Wei, YT.,Liu, R.,...&高学云.(2013).Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels.ANALYST,138(8),2302-2307.
MLA 魏月腾,et al."Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels".ANALYST 138.8(2013):2302-2307.
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