The structural basis of the response regulator DrRRA from Deinococcus radiodurans
Liu Y(刘颖); Gao ZQ(高增强); She Z(佘准); Liu, Y; Gao, ZQ; She, Z; Qu, K; Wang, WJ; Shtykova, EV; Xu, JH; Ji, CN; Dong, YH; Qu K(曲昆); Wang WJ(王文佳); Xu JH(徐建华); Dong YH(董宇辉)
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2012
卷号417期号:4页码:1206-1212
关键词Deinococcus radiodurans Two component system Response regulator Extensive interdomain interface DNA-binding Crystal structure SAXS
学科分类Biochemistry & Molecular Biology; Biophysics
DOI10.1016/j.bbrc.2011.12.110
英文摘要DrRRA, a vital and recently discovered gene product of Deinococcus radiodurans, is a member of the OmpR/PhoB family of response regulators that couple with the cognate histidine kinase (HK) to form a typical two component system (TCS). It is known that the DrRRA is responsible for the transcriptional levels of numerous genes mostly relating to the stress response and DNA repair. In this paper, the crystal structures of the effector domain and full-length protein of DrRRA with resolutions of 1.6 and 2.3 angstrom, respectively, are determined. These crystal structures depicted that DrRRA has the structural features of the OmpR/PhoB subfamily and were also confirmed by SAXS investigation of the protein in solution. Our data suggest that the receiver domain blocks the binding of DNA to the DNA recognition helix of effector domain; while the interdomain interface would be unwrapped, via the phosphorylation of receiver domain and/or the inducement of DNA, in order to provide DNA binding. (C) 2011 Elsevier Inc. All rights reserved.
收录类别SCI
WOS记录号WOS:000300196100017
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224117
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
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Liu Y,Gao ZQ,She Z,et al. The structural basis of the response regulator DrRRA from Deinococcus radiodurans[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2012,417(4):1206-1212.
APA 刘颖.,高增强.,佘准.,Liu, Y.,Gao, ZQ.,...&董宇辉.(2012).The structural basis of the response regulator DrRRA from Deinococcus radiodurans.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,417(4),1206-1212.
MLA 刘颖,et al."The structural basis of the response regulator DrRRA from Deinococcus radiodurans".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 417.4(2012):1206-1212.
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