Structure-function studies of Escherichia coli RnlA reveal a novel toxin structure involved in bacteriophage resistance
Wei Y(魏勇); Gao ZQ(高增强); Wei, Y; Gao, ZQ; Otsuka, Y; Naka, K; Yonesaki, T; Zhang, H; Dong, YH; Dong YH(董宇辉); Zhang H(张衡)
刊名MOLECULAR MICROBIOLOGY
2013
卷号90期号:5页码:956-965
学科分类Biochemistry & Molecular Biology; Microbiology
DOI10.1111/mmi.12409
英文摘要Escherichia coli RnlA-RnlB is a newly identified toxin-antitoxin (TA) system that plays a role in bacteriophage resistance. RnlA functions as a toxin with mRNA endoribonuclease activity and the cognate antitoxin RnlB inhibits RnlA toxicity in E. coli cells. Interestingly, T4 phage encodes the antitoxin Dmd, which acts against RnlA to promote its own propagation, suggesting that RnlA-Dmd represents a novel TA system. Here, we have determined the crystal structure of RnlA refined to 2.10 angstrom. RnlA is composed of three independent domains: NTD (N-terminal domain), NRD (N repeated domain) and DBD (Dmd-binding domain), which is an organization not previously observed among known toxin structures. Small-angle X-ray scattering (SAXS) analysis revealed that RnlA forms a dimer in solution via interactions between the DBDs from both monomers. The in vitro and in vivo functional studies showed that among the three domains, only the DBD is responsible for recognition and inhibition by Dmd and subcellular location of RnlA. In particular, the helix located at the C-terminus of DBD plays a vital role in binding Dmd. Our comprehensive studies reveal the key region responsible for RnlA toxicity and provide novel insights into its structure-function relationship.
收录类别SCI
WOS记录号WOS:000327374300004
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224085
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
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Wei Y,Gao ZQ,Wei, Y,et al. Structure-function studies of Escherichia coli RnlA reveal a novel toxin structure involved in bacteriophage resistance[J]. MOLECULAR MICROBIOLOGY,2013,90(5):956-965.
APA 魏勇.,高增强.,Wei, Y.,Gao, ZQ.,Otsuka, Y.,...&张衡.(2013).Structure-function studies of Escherichia coli RnlA reveal a novel toxin structure involved in bacteriophage resistance.MOLECULAR MICROBIOLOGY,90(5),956-965.
MLA 魏勇,et al."Structure-function studies of Escherichia coli RnlA reveal a novel toxin structure involved in bacteriophage resistance".MOLECULAR MICROBIOLOGY 90.5(2013):956-965.
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