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Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics
Gao YX(高愈希); Peng XM(彭晓敏); Zhao JT(赵甲亭); Gao, YX; Peng, XM; Zhang, JC; Zhao, JT; Li, YY; Li, YF; Li, B; Hu, Y; Chai, ZF;; Li YY(李云云); Li YF(李玉锋); Li B(李柏); Hu Y(胡毅); Chai ZF(柴之芳)
2013
发表期刊METALLOMICS
卷号5期号:7页码:913-919
摘要The response of E. coli to Hg2+ exposure was investigated using proteomic and metalloproteomic approaches. E. coli was cultured in the LB medium containing HgCl2 and/or selenomethionine. The growth curve of E. coli was measured to estimate the toxicity of Hg2+ or selenomethionine. After two-dimensional gel electrophoresis (2-DE), distribution of Hg in 2-DE gel was detected with synchrotron radiation X-ray fluorescence (SRXRF) at 4W1B, Beijing Synchrotron Radiation Facility. The proteins with differential expression and those containing Hg were identified with electrospray ionization tandem mass spectrometry (ESI-MS/MS) and peptide mass fingerprinting analysis. The results showed that Hg2+ can inhibit the growth of E. coli, while supplement of selenomethionine can shorten the lag period induced by Hg2+, indicating an antagonistic effect of selenomethionine against Hg2+ toxicity. Mechanistically, Hg was observed to be able to bind pyruvate kinase, a glycolytic enzyme, and modulate the expression of five other proteins, including down-regulation of outer membrane protein W and up-regulation of transcription termination factor rho, cysteine synthase, transaldolase A and alkyl hydroperoxide reductase subunit C. Therefore, our results indicated that mercury may influence osmosis of plasma membrane, antioxidant defense, and glycometabolism of the microorganism. This study demonstrates the high sensitivity of SRXRF in identifying metal-associated proteins compared to conventional proteomic approaches.
学科领域Biochemistry & Molecular Biology
DOI10.1039/c3mt20279h
收录类别SCI
WOS记录号WOS:000320951600018
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224082
专题院士
实验物理中心
多学科研究中心
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GB/T 7714
Gao YX,Peng XM,Zhao JT,et al. Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics[J]. METALLOMICS,2013,5(7):913-919.
APA 高愈希.,彭晓敏.,赵甲亭.,Gao, YX.,Peng, XM.,...&柴之芳.(2013).Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics.METALLOMICS,5(7),913-919.
MLA 高愈希,et al."Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics".METALLOMICS 5.7(2013):913-919.
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