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Cloning, purification, crystallization and preliminary X-ray studies of human a1-microglobulin
Zhang, YL; Gao ZQ(高增强); Gao, ZQ; Zhang, ZZ; Luo, M; Huang, AL; Dong, YH; Wang, DQ;董宇辉
2012
发表期刊ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
卷号68期号:6页码:692-694
摘要a1-Microglobulin (a1m) is one of the phylogenetically most widespread lipocalins and is distributed in various organs and tissues, including liver, heart, eye, kidney, brain, lung, pancreas and skeletal muscle. a1m has been found to exert multifarious functions, including interacting with IgA, albumin and prothrombin, binding strongly to haem and exhibiting reductase activity. Nevertheless, little structural information is available regarding these functions of a1m. Since determination of three-dimensional structure is a powerful means of functional characterization, X-ray crystallography was used to accomplish this task. Here, the expression, purification, crystallization and preliminary crystallographic analysis of human a1m are reported. The crystal belonged to space group P43, with unit-cell parameters a = b = 36.45, c = 112.68 angstrom, and diffracted to a resolution of 2.0 angstrom. The crystals are most likely to contain one molecule in the asymmetric unit, with a VM value of 1.63 angstrom 3 Da-1.
关键词a1-microglobulin lipocalins
学科领域Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S1744309112016569
收录类别SCI
WOS记录号WOS:000305073600019
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224041
专题多学科研究中心
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GB/T 7714
Zhang, YL,Gao ZQ,Gao, ZQ,et al. Cloning, purification, crystallization and preliminary X-ray studies of human a1-microglobulin[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2012,68(6):692-694.
APA Zhang, YL.,高增强.,Gao, ZQ.,Zhang, ZZ.,Luo, M.,...&Wang, DQ;董宇辉.(2012).Cloning, purification, crystallization and preliminary X-ray studies of human a1-microglobulin.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,68(6),692-694.
MLA Zhang, YL,et al."Cloning, purification, crystallization and preliminary X-ray studies of human a1-microglobulin".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 68.6(2012):692-694.
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