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Purification, crystallization and preliminary crystallographic analysis of the 23S rRNA methyltransferase RlmM (Cm2498) from Escherichia coli
Guo, HY; Gao ZQ(高增强); Zhang H(张衡); Gao, ZQ; Zhang, H; Wei, Y; Xu, JH; Wang, WY; Yan, AX; Dong, YH;; Wei Y(魏勇); Xu JH(徐建华); Dong YH(董宇辉)
2013
发表期刊ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
卷号69期号:6页码:640-642
摘要RlmM is an AdoMet-dependent methyltransferase that is responsible for 2'-O-methylation of C2498 in the peptidyl-transferase loop of bacterial 23S rRNA. This modification occurs before assembly of the 50S ribosomal subunit, and lack of C2498 methylation can cause a slight reduction in bacterial fitness. Here, the purification and crystallization of RlmM from Escherichia coli as well as its preliminary crystallographic analysis are presented. Cocrystallization of RlmM with AdoMet was carried out and X-ray diffraction data were collected to a resolution of 2.30 angstrom on beamline BL17U at the SSRF. However, electron density for AdoMet cannot be observed by comprehensive crystallographic analysis, indicating that it is not bound by RlmM during the cocrystallization process. The structure was solved by molecular replacement and refinement is in progress. The crystal contained one molecule in the asymmetric unit and belonged to space group P2(1), with unit-cell parameters a = 56.07, b = 59.38, c = 54.35 angstrom, beta = 94.84 degrees, which differs from the P3(1) or P3(1)21 space groups of previously reported RlmM structures (PDB entries 4auk, 4atn and 4b17).
学科领域Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S1744309113006611
收录类别SCI
WOS记录号WOS:000319736000010
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文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224036
专题多学科研究中心
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Guo, HY,Gao ZQ,Zhang H,et al. Purification, crystallization and preliminary crystallographic analysis of the 23S rRNA methyltransferase RlmM (Cm2498) from Escherichia coli[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69(6):640-642.
APA Guo, HY.,高增强.,张衡.,Gao, ZQ.,Zhang, H.,...&董宇辉.(2013).Purification, crystallization and preliminary crystallographic analysis of the 23S rRNA methyltransferase RlmM (Cm2498) from Escherichia coli.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,69(6),640-642.
MLA Guo, HY,et al."Purification, crystallization and preliminary crystallographic analysis of the 23S rRNA methyltransferase RlmM (Cm2498) from Escherichia coli".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 69.6(2013):640-642.
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