Subacute toxicity of 14 nm nanoparticulate silver (Ag-NP) stabilised with polyvinylpyrrolidone and ionic silver in the form of silver acetate (Ag-acetate) was investigated in four-week-old Wistar rats. Animals received orally by gavage the following: vehicle control (10 a (TM) Euro, 6 a (TM),); Ag-NP at doses: 2.25 (8 a (TM) Euro), 4.5 (8 a (TM) Euro) or 9 mg/kg bw/day (10 a (TM) Euro, 6 a (TM),); or Ag-acetate 9 mg silver/kg bw/day (8 a (TM) Euro) for 28 days. Clinical, haematolological and biochemical parameters, organ weights, macro- and microscopic pathological changes were investigated. Caecal bacterial phyla and their silver resistance genes were quantified. For the Ag-NP groups, no toxicological effects were recorded. For Ag-acetate, lower body weight gain (day 4-7, 11-14, 14-16, P < 0.05; overall, day 1-28, P < 0.01), increased plasma alkaline phosphatase (P < 0.05), decreased plasma urea (P < 0.05) and lower absolute (P < 0.01) and relative (P < 0.05) thymus weight were recorded. In conclusion, these findings indicate toxicity of 9 mg/kg bw/day ionic silver but not of an equimolar Ag-NP dose. This is in accordance with previously reported data showing that oral Ag-acetate, in comparison with an equimolar dose of Ag-NP, resulted in higher silver plasma and organ concentrations.
Hadrup, N,Loeschner, K,Bergstrom, A,et al. Subacute oral toxicity investigation of nanoparticulate and ionic silver in rats[J]. ARCHIVES OF TOXICOLOGY,2012,86(4):543-551.
Hadrup, N.,Loeschner, K.,Bergstrom, A.,Wilcks, A.,Gao, XY.,...&Mortensen, A；高学云.(2012).Subacute oral toxicity investigation of nanoparticulate and ionic silver in rats.ARCHIVES OF TOXICOLOGY,86(4),543-551.
Hadrup, N,et al."Subacute oral toxicity investigation of nanoparticulate and ionic silver in rats".ARCHIVES OF TOXICOLOGY 86.4(2012):543-551.