Separation and purification of fullerenols for improved biocompatibility
Li J(李娟); Zhang MY(张铭倚); Sun BY(孙宝云); Li, J; Zhang, MY; Sun, BY; Xing, GM; Song, Y; Guo, HL; Chang, YA; Ge, YH; Zhao, YL;; Xing GM(邢更妹); Song Y(宋炎); Chang YN(常亚男)
刊名CARBON
2012
卷号50期号:2页码:460-469
学科分类Chemistry; Materials Science
DOI10.1016/j.carbon.2011.08.073
英文摘要Isoelectric focusing was used to separate the different components of fullerenols based on their different electronegativities induced by the surface chemical functionalities and structures. Purified fractions of fullerenols were analyzed and characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results showed that four fractions of fullerenols could be separated by isoelectric focusing, and all fractions had a general formula of C-60(OH)(m)(O)(n) where the values of m and n might vary with their isoelectric points. Results from the capillary electrophoresis studies showed that the negative surface charge densities were different among the fractions of fullerenols, which indicated that the net surface negative charge was determined by the degree of C=O functionalization. In vitro cytotoxicity assays were also performed to determine the effect of purified fullerenols on cytotoxicity and DNA stability in Raw 264.7 cells. Although the fullerenols had low cytotoxicity, the composition of fullerenols with the highest negative charge density (-1.913 +/- 0.008 x 10(-4) C) and smaller size (3.92 +/- 0.71 nm) was found to induce DNA damage. Therefore, crude fullerenols must be separated and purified in order to obtain fullerenols with low degree of C=O functionalization and good biocompatibility. (C) 2011 Elsevier Ltd. All rights reserved.
收录类别SCI
WOS记录号WOS:000297397700014
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/223988
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
推荐引用方式
GB/T 7714
Li J,Zhang MY,Sun BY,et al. Separation and purification of fullerenols for improved biocompatibility[J]. CARBON,2012,50(2):460-469.
APA 李娟.,张铭倚.,孙宝云.,Li, J.,Zhang, MY.,...&常亚男.(2012).Separation and purification of fullerenols for improved biocompatibility.CARBON,50(2),460-469.
MLA 李娟,et al."Separation and purification of fullerenols for improved biocompatibility".CARBON 50.2(2012):460-469.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
4152.pdf(1164KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[李娟]的文章
[张铭倚]的文章
[孙宝云]的文章
百度学术
百度学术中相似的文章
[李娟]的文章
[张铭倚]的文章
[孙宝云]的文章
必应学术
必应学术中相似的文章
[李娟]的文章
[张铭倚]的文章
[孙宝云]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 4152.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。