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Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa
Wei Y(魏勇);  Zhang H(张衡);  Gao ZQ(高增强);  Wei, Y; Zhang, H; Gao, ZQ; Xu, JH; Liu, QS; Dong, YH; Xu JH(徐建华);  Liu QS(刘全胜);  Dong YH(董宇辉)
2013
发表期刊IUBMB LIFE
卷号65期号:SI1页码:50-57
摘要The global metabolic regulator catabolite repression control (Crc) has recently been found to modulate the susceptibility to antibiotics and virulence in the opportunistic pathogen Pseudomonas aeruginosa and been suggested as a nonlethal target for novel antimicrobials. In P. aeruginosa, Crc couples with the CA motifs from the small RNA CrcZ to form a post-transcriptional regulator system and is removed from the 5'-end of the target mRNAs. In this study, we first reported the crystal structure of Crc from P. aeruginosa refined to 2.20 angstrom. The structure showed that it consists of two halves with similar overall topology and there are 11 beta strands surrounded by 13 helices, forming a four-layered a/beta-sandwich. The circular dichroism spectroscopy revealed that it is thermostable in solution and shares similar characteristics to that in crystal. Comprehensive structural analysis and comparison with the homologies of Crc showed high similarity with several known nucleases and consequently may be classified into a member exodeoxyribonuclease III. However, it shows distinct substrate specificity (RNA as the preferred substrate) compared to these DNA endonucleases. Structural comparisons also revealed potential RNA recognition and binding region mainly consisting of five flexible loops. Our structure study provided the basis for the future application of Crc as a target to develop new antibiotics. (c) 2012 IUBMB Life, 65(1):5057, 2013
关键词global metabolic regulator Pseudomonas aeruginosa RNA binding crystal structure target for antimicrobials
学科领域Biochemistry & Molecular Biology; Cell Biology
DOI10.1002/iub.1103
收录类别SCI
WOS记录号WOS:000312886200008
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/223798
专题多学科研究中心
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GB/T 7714
魏勇;张衡;高增强;Wei, Y,Zhang, H,Gao, ZQ,et al. Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa[J]. IUBMB LIFE,2013,65(SI1):50-57.
APA 魏勇;张衡;高增强;Wei, Y.,Zhang, H.,Gao, ZQ.,Xu, JH.,Liu, QS.,...&徐建华;刘全胜;董宇辉.(2013).Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa.IUBMB LIFE,65(SI1),50-57.
MLA 魏勇;张衡;高增强;Wei, Y,et al."Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa".IUBMB LIFE 65.SI1(2013):50-57.
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